Anti-HIF-1 alpha Rabbit pAb
- 100 μL
Product Information
| Description |
HIF-1 alpha rabbit polyclonal |
| Protein full name |
Hypoxia-inducible factor 1-alpha |
|
Synonyms |
HIF1A, HIF-1-alpha, HIF-1A, HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78, hypoxia inducible factor 1 alpha subunit |
| Immunogen |
KLH conjugated Synthetic peptide corresponding to Mouse HIF-1α |
| Isotype | IgG |
| Purity | Affinity purification |
| Predicted MW. |
93/120KDa |
| Observed MW. |
93KDa |
| Uniprot ID |
Applications
Applications | Species | Dilution | Positive Tissue |
WB | Human, Mouse, Rat | 1:500-1:1000 | Hela,HepG2,HCT116,lung,brain |
Background
Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBBP and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia.
Images
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Western blot analysis of HIF-1α(GB11031) at dilution of 1:1000. Lane 1:Hela cell lysate Lane 2:Hela cell lysate (treated with COCL2) Lane 3:HepG2 cell lysate Lane 4:HCT116 cell lysate Lane 5:Mouse lung tissue lysate Lane 6:Mouse brain tissue lysate Lane 7:Rat lung tissue lysate Lane 8:Rat brain tissue lysate |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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