产品信息

蛋白质全称	干扰素诱导GTP结合蛋白Mx1
别名	Influenza resistance protein, Myxoma resistance protein 1, Myxovirus resistance protein 1, Mx1, Mx, IFI78K
Uniprot ID	Q3UD61
免疫原	KLH偶联的小鼠MX1合成肽
抗体亚型	IgG
预测分子量./观测分子量.	72 kDa / 72 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	人, 小鼠, 大鼠	1: 800-1: 1600	肾, 肝

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背景

The Dynamin family of microtubule-associated proteins function as GTPases that are involved in microtubule bundling and endocytosis. In mice, Mx1 (myxovirus resistance protein 1) is member of the Dynamin family that is involved in the immune response to viral infections. Localized to the cytoplasm, Mx1 is localized to the nucleus where, upon induction by IFN-alpha/IFN-beta, it provides selective resistance to infection by the highly lethal H5N1 influenza virus.

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图像

	WB检测MX1蛋白(货号 GB112553). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 800稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.
	WB检测MX1蛋白(货号 GB112553). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 800稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.