产品信息

蛋白质全称	神经酰胺合酶4
别名	CerS4, LAG1 longevity assurance homolog 4, Sphingosine N-acyltransferase CERS4, Translocating chain-associating membrane protein homolog 1, TRAM homolog 1, Lass4, Trh1
Uniprot ID	Q9D6J1
免疫原	小鼠LASS4重组蛋白
抗体亚型	IgG
预测分子量./观测分子量.	46 kDa / 46 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	人, 小鼠, 大鼠	1: 500-1: 1000	胚胎, 心, 肺

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背景

LASS4 is a member of the LASS (longevity assurance homologue) family which are involved in determining life span in yeast, by an unknown molecular mechanism. Members of this family are highly conserved from yeasts to mammals.LASS proteins are thought to be involved in ceramide synthesis. Overproduction of a single LASS protein results in an increase in one or more specific ceramide species, which differ in fatty-acid chain length.

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图像

WB检测LASS4蛋白(货号 GB112934). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 1000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.