产品信息

蛋白质全称	二磷酸肌醇多磷酸磷酸水解酶3β
别名	DIPP-3-beta, DIPP3-beta, Diadenosine 5',5'''-P1,P6-hexaphosphate hydrolase 3-beta, Diadenosine hexaphosphate hydrolase (AMP-forming), Nudix motif 11, Nudt11, Dipp3b, MNCb-1696, Nucleoside diphosphate-linked moiety X motif 11
Uniprot ID	P0C027
免疫原	小鼠NUDT11重组蛋白
抗体亚型	IgG
预测分子量./观测分子量.	19 kDa / 19 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	人, 小鼠	1: 300-1: 600	肾, 睾丸, 脑

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背景

Cleaves a beta-phosphate from the diphosphate groups in PP-InsP5 (diphosphoinositol pentakisphosphate), suggesting that it may play a role in signal transduction. Also able to catalyze the hydrolysis of dinucleoside oligophosphates, with Ap6A and Ap5A being the preferred substrates. The major reaction products are ADP and p4a from Ap6A and ADP and ATP from Ap5A. Also able to hydrolyze 5-phosphoribose 1-diphosphate; however, the relevance of such activity in vivo remains unclear.

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图像

	WB检测NUDT11蛋白(货号 GB112456). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 300稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.
	WB检测NUDT11蛋白(货号 GB112456). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 300稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.