产品信息

蛋白质全称	硫氧还蛋白
别名	Surface associated sulphydryl protein, TRDX, Thioredoxin1, DKFZp686B1993, MGC61975, Trx, ATL-derived factor, ADF, Txn, Txn1, SASP, ATL-derived factor, zgc:92903, TXN,  TRX, TRX1, Thioredoxin, ATL-derived factor, TRDX, Surface-associated sulphydryl protein, Hom s Trx
Uniprot ID	P10639
免疫原	小鼠Thioredoxin重组蛋白
抗体亚型	IgG
预测分子量./观测分子量.	12 kDa / 12 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	小鼠, 大鼠	1: 500-1: 1000	结肠, 肺, 肝

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背景

Thioredoxins (Trx) are small, multi-functional proteins with oxidoreductase activity and are ubiquitous in essentially all living cells. Trx contains a redox-active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. The two cystein residues in the conserved active centers can be oxidized to form intramolecular disulfide bonds. Reduction of the active site disulfide in oxidized Trx is catalyzed by Trx reductase with NADPH as the electron donor.

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图像

	WB检测Thioredoxin/TRX蛋白(货号 GB11993). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.
	WB检测Thioredoxin/TRX蛋白(货号 GB11993). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.