产品信息

蛋白质全称	E3 SUMO连接酶PIAS4
别名	PIASy, Protein inhibitor of activated STAT protein 4, PIAS-gamma, PIAS4, Piasg, ZMIZ6, PIAS4
Uniprot ID	Q9JM05
免疫原	KLH偶联的小鼠E3 SUMO-protein ligase PIAS4合成肽
抗体亚型	IgG
预测分子量./观测分子量.	56 kDa / 72 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	人, 大鼠	1: 500-1: 1000	睾丸

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背景

PIAS4(protein inhibitor of activated STAT protein 4) is also named as PIASG, PIASy and belongs to the PIAS family. It enhanced the conjugation of SUMO2 to GATA2 through its E3 SUMO ligase activity. TGF-b can up-regulates PLAS4 expression in MCs to down-regulatea-SMA gene transcription by the interaction with E12 and PLAS4 predominantly acts as E3 ligases for E12 in MCs among PIAS family members . This protein can be sumoylated and Lys-35 is the main site of sumoylation.

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图像

WB检测E3 SUMO-protein ligase PIAS4/PIASy蛋白(货号 GB113100). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 1000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.