产品信息

蛋白质全称	E3泛素连接酶TRAF3IP2
别名	ACT1, Adapter protein CIKS, C6orf2, C6orf4, C6orf5, C6orf6, CIKS, Connection to IKK and SAPK/JNK, TRAF3 interacting protein 2, TRAF3IP2
Uniprot ID	Q8N7N6
免疫原	小鼠CIKS/ACT1重组蛋白
抗体亚型	IgG
纯化方式	亲和纯化
亚细胞定位	细胞核, 细胞质, 分泌型

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应用

应用	物种	稀释	阳性样品
IHC/IF 免疫组织化学/免疫荧光	大鼠	1: 1800-1: 3600	脾

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背景

This gene encodes a protein involved in regulating responses to cytokines by members of the Rel/NF-kappaB transcription factor family. These factors play a central role in innate immunity in response to pathogens, inflammatory signals and stress. This gene product interacts with TRAF proteins (tumor necrosis factor receptor-associated factors) and either I-kappaB kinase or MAP kinase to activate either NF-kappaB or Jun kinase. Several alternative transcripts encoding different isoforms have been identified. Another transcript, which does not encode a protein and is transcribed in the opposite orientation, has been identified.

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图像

IHC检测CIKS/ACT1蛋白(货号 GB113480). 样品: 大鼠脾, 4%多聚甲醛 (货号G1101) 固定12-24小时. 抗原修复: 柠檬酸抗原修复液(干粉, pH 6.0) (G1201), 98℃, 20分钟. —抗: 1: 1800稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 200稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.