产品信息

蛋白质全称	E3泛素连接酶CHIP
别名	Antigen NY-CO-7, CHIP, CLL associated antigen KW 8, HSPABP2, NY CO 7, SDCCAG7, STUB1, UBOX1, PP1131, Antigen NY CO 7, Carboxy terminus of Hsp70 interacting protein, C terminus of Hsp70-interacting protein
Uniprot ID	Q9WUD1
免疫原	KLH偶联的小鼠STUB1合成肽
抗体亚型	IgG
预测分子量./观测分子量.	35 kDa / 35 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	人, 小鼠, 大鼠	1: 1000-1: 3000	肺, 心

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背景

STUB1, also named as CHIP, SDCCAG7, UBOX1 and NY-CO-7, is a E3 ubiquitin-protein ligase which targets misfolded chaperone substrates towards proteasomal degradation. STUB1/CHIP modulates the activity of several chaperone complexes, including Hsp70, Hsc70 and Hsp90. It mediates transfer of non-canonical short ubiquitin chains to HSPA8 that have no effect on HSPA8 degradation. STUB1/CHIP mediates polyubiquitination of CYP3A4. CHIP/STUB1 strongly reduced keratin aggregates through increased degradation of mutant K14.

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图像

	WB检测STUB1/CHIP蛋白(货号 GB113826). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 3000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.
	WB检测STUB1/CHIP蛋白(货号 GB113826). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 3000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.