Anti-Cleaved-Caspase-3 Rabbit pAb
- 100 μL
Product Information
Description | Cleaved- Caspase-3 rabbit polyclonal |
Protein full name | Caspase-3 |
Synonyms | CASP3, CPP32, CPP32B, SCA-1, caspase 3 |
Immunogen | KLH conjugated Synthetic peptide corresponding to Mouse caspase3 |
Isotype | IgG |
Predicted MW. | 17/35KDa |
Observed MW. | 17/35KDa |
Purity | Affinity purification |
Subcellular location | |
Uniprot ID |
Applications
applications | species | dilution | Positive tissue |
WB | Human, Mouse, Rat | 1: 1000 | Hela, HepG2, SW480 |
IHC | Mouse | 1: 200-1: 1000 | tumor |
IF | Mouse | 1: 200-1: 1000 | tumor |
Background
Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Triggers cell adhesion in sympathetic neurons through RET cleavage (By similarity). Cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes.
Images
| Western blot analysis of Cleaved-Caspase3 (GB11009) at dilution of 1:1000. Lane 1:Hela cell lysate Lane 2:HepG2 cell lysate Lane 3:SW480 cell lysate Lane 4:B16 cell lysate Lane 5:C6 cell lysate |
 | Immunohistochemistry analysis of paraffin-embedded mouse tumor using Cleaved-Caspase3 (GB11009) at dilution of 1:1000 |
 | Immunofluorescence analysis of paraffin-embedded mouse tumor using Cleaved-Caspase3 (GB11009) at dilution of 1:500 |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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