产品信息

蛋白质全称	BAG家族分子伴侣调节因子1
别名	BAG 1, BAG1, BAG1L speicfic, BAG1M, BAG1S, HAP, RAP46
Uniprot ID	Q60739
免疫原	KLH偶联的小鼠Bag1合成肽
抗体亚型	IgG
纯化方式	亲和纯化
亚细胞定位	细胞核, 细胞质

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应用

应用	物种	稀释	阳性样品
IHC/IF 免疫组织化学/免疫荧光	小鼠, 大鼠	1: 150-1: 600	睾丸

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背景

Co-chaperone for HSP70 and HSC70 chaperone proteins. Acts as a nucleotide-exchange factor promoting the release of ADP from the HSP70 and HSC70 proteins thereby triggering client/substrate protein release. Nucleotide release is mediated via its binding to the nucleotide-binding domain of HSPA8/HSC70 where as the substrate release is mediated via its binding to the substrate-binding domain of HSPA8/HSC70. Inhibits the pro-apoptotic function of PPP1R15A, and has anti-apoptotic activity. Markedly increases the anti-cell death function of BCL2 induced by various stimuli.

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图像

	IHC检测Bag1蛋白(货号 GB115597). 样品: 小鼠睾丸, 4%多聚甲醛 (货号G1101) 固定12-24小时. 抗原修复: Tris-EDTA抗原修复液(pH 9.0) (G1203), 98℃, 20分钟. —抗: 1: 150稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 200稀释, 室温孵育1小时.
	IHC检测Bag1蛋白(货号 GB115597). 样品: 大鼠睾丸, 4%多聚甲醛 (货号G1101) 固定12-24小时. 抗原修复: Tris-EDTA抗原修复液(pH 9.0) (G1203), 98℃, 20分钟. —抗: 1: 150稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 200稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.