产品信息

蛋白质全称	含杆状病毒IAP重复序列的蛋白3
别名	Cellular inhibitor of apoptosis 2, C-IAP2, Birc3, Inhibitor of apoptosis protein 1, mIAP1, RING-type E3 ubiquitin transferase BIRC3
Uniprot ID	O08863
免疫原	小鼠cIAP2重组蛋白
抗体亚型	IgG
预测分子量./观测分子量.	67 kDa / 67 kDa
纯化方式	亲和纯化

---

应用

应用	物种	稀释	阳性样品
WB 免疫印迹	人, 小鼠	1: 500-1: 1000	Raji细胞, HepG2细胞, 293T细胞

---

背景

Multi-functional protein which regulates not only caspases and apoptosis, but also modulates inflammatory signaling and immunity, mitogenic kinase signaling and cell proliferation, as well as cell invasion and metastasis. Acts as an E3 ubiquitin- protein ligase regulating NF-kappa-B signaling and regulates both canonical and non-canonical NF-kappa-B signaling by acting in opposite directions: acts as a positive regulator of the canonical pathway and suppresses constitutive activation of non-canonical NF-kappa-B signaling.

---

图像

WB检测cIAP2蛋白(货号 GB11892). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.

---

储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

---

注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.