产品信息

蛋白质全称	受体相互作用丝氨酸/苏氨酸蛋白激酶3
别名	Receptor interacting protein 3, RIP 3, RIP like protein kinase 3, RIP3, RIPK3, Rip3 , KIAA0864, M RIP, MPRIP, MRIP, p116Rip, Rho interacting protein 3, RHOIP3
Uniprot ID	Q9Y572
免疫原	KLH偶联的人RIP3合成磷酸肽
抗体亚型	IgG
预测分子量./观测分子量.	57 kDa / 57 kDa
纯化方式	亲和纯化

---

应用

应用	物种	稀释	阳性样品
WB 免疫印迹	人	1: 300-1: 500	THP-1细胞, HT-29细胞, Jurkat细胞, SK-BR-3细胞

---

背景

Serine/threonine-protein kinase that activates necroptosis and apoptosis, two parallel forms of cell death.Necroptosis, a programmed cell death process in response to death-inducing TNF-alpha family members, is triggered by RIPK3 following activation by ZBP1.Activated RIPK3 forms a necrosis-inducing complex and mediates phosphorylation of MLKL, promoting MLKL localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage.In addition to TNF-induced necroptosis, necroptosis can also take place in the nucleus in response to orthomyxoviruses infection: following ZBP1 activation, which senses double-stranded Z-RNA structures, nuclear RIPK3 catalyzes phosphorylation and activation of MLKL, promoting disruption of the nuclear envelope and leakage of cellular DNA into the cytosol.

---

图像

WB检测RIP3蛋白(货号 GB115458). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 300稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.

---

储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

---

注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.