产品信息

蛋白质全称	mCherry荧光蛋白
别名	Monomeric cherry red fluorescent protein
Uniprot ID	X5DSL3
免疫原	mCherry重组蛋白
抗体亚型	IgG2a, κ
克隆号	4D8A8
预测分子量./观测分子量.	32 kDa / 32 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	Species independent	1: 500-1: 1000	mCherry重组蛋白
IHC/IF 免疫组织化学/免疫荧光	Species independent	1: 500-1: 1500	mCherry重组蛋白

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背景

mCherry is derived from proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals), and is used as a fluorescent tracer in trasfection and transgenic experiments. The prototype for these fluorescent proteins is Green Fluorescent Protein (GFP), which is a ~27kDa protein isolated originally from the jellyfish Aequoria victoria. The mCherry protein is derived from DsRed, a red fluorescent protein related to GFP isolated from so-called disc corals of the genus Discosoma.

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图像

WB检测mCherry蛋白(货号 GB122058). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 1000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗小鼠IgG (H+L) (货号GB23301), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.