产品信息

蛋白质全称	CMRF35样分子8
别名	CLM-8, CD300 antigen-like family member A, Leukocyte mono-Ig-like receptor 1, Myeloid-associated immunoglobulin-like receptor 1, MAIR-1, CD300a, Clm8, Lmir1, Mcpir1
Uniprot ID	Q6SJQ0
免疫原	小鼠CD300a重组蛋白
抗体亚型	IgG
预测分子量./观测分子量.	36 kDa / 36 kDa
纯化方式	亲和纯化

---

应用

应用	物种	稀释	阳性样品
WB 免疫印迹	人	1: 500-1: 1000	Jurkat细胞, K562细胞, Raji细胞

---

背景

The CMRF35 antigen, which was identified by reactivity with a monoclonal antibody, is present on monocytes, neutrophils, and some T and B lymphocytes. CMRF35H is recognized by the same antibody and is distinct from CMRF35. Inhibitory receptor which may contribute to the down-regulation of cytolytic activity in natural killer (NK) cells, and to the down-regulation of mast cell degranulation.

---

图像

WB检测CD300a蛋白(货号 GB111832). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 1000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.

---

储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

---

注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.