产品信息

蛋白质全称	突出蛋白-1
别名	AC133, Antigen AC133, CD133, CORD12, MCDR2, MSTP061, PROM1, Prominin like protein 1, PROML1, RP41, STGD4, STGD4, Hematopoietic stem cell antigen, prominin 1
Uniprot ID	O54990
免疫原	KLH偶联的小鼠CD133合成肽
抗体亚型	IgG1, κ
克隆号	9G9D8
纯化方式	亲和纯化
亚细胞定位	细胞膜

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应用

应用	物种	稀释	阳性样品
IHC/IF 免疫组织化学/免疫荧光	大鼠	1: 200-1: 800	胎鼠

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背景

CD133, also known as PROM1 (prominin-1) or AC133, belongs to the prominin family. CD133 is a transmembrane glycoprotein with an NH2-terminal extracellular domain, five transmembrane loops and a cytoplasmic tail. The expression of CD133 has been reported in hematopoietic stem cells, endothelial progenitor cells, neuronal and glial stem cells, suggesting the potential role of CD133 as a cell surface marker of adult stem cells. CD133 has also been reported as a marker of cancer stem cells in various human tumors.

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图像

	IHC检测CD133蛋白(货号 GB12060). 样品: 胎鼠, 4%多聚甲醛 (货号G1101) 固定12-24小时. 抗原修复: 柠檬酸抗原修复液(干粉, pH 6.0) (G1201), 高压锅均匀喷气计时2分钟. —抗: 1: 400稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗小鼠IgG (H+L) (货号GB23301), 1: 200稀释, 室温孵育1小时.
	IHC检测CD133蛋白(货号 GB12060). 样品: 胎鼠, 4%多聚甲醛 (货号G1101) 固定12-24小时. 抗原修复: 柠檬酸抗原修复液(干粉, pH 6.0) (G1201), 高压锅均匀喷气计时2分钟. —抗: 1: 400稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗小鼠IgG (H+L) (货号GB23301), 1: 200稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.