产品信息

蛋白质全称	多萜醇二磷酸寡糖蛋白糖基转移酶STT3B
别名	Oligosaccharyl transferase subunit STT3B, STT3-B, B6dom1 antigen, Source of immunodominant MHC-associated peptides, Stt3b, Simp
Uniprot ID	Q3TDQ1
免疫原	小鼠STT3B重组蛋白
抗体亚型	IgG
预测分子量./观测分子量.	93 kDa / 100 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	人	1: 2000-1: 4000	HeLa细胞, A431细胞, U2OS细胞

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背景

STT3B is a component of the N-oligosaccharyl transferase (OST) enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). SST3B seems to be involved in complex substrate specificity.

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图像

WB检测STT3B蛋白(货号 GB112753). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 2000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.