产品信息

蛋白质全称	1型半胱氨酸双加氧酶
别名	CDO, CDO-I, CDO1, Cysteine dioxygenase type 1, cysteine dioxygenase, type I
Uniprot ID	P60334
免疫原	KLH偶联的小鼠Cysteine Dioxygenase CDO1合成肽
抗体亚型	IgG
预测分子量./观测分子量.	23 kDa / 23 kDa
纯化方式	亲和纯化

---

应用

应用	物种	稀释	阳性样品
WB 免疫印迹	小鼠, 大鼠	1: 1500-1: 3000	肝, 肺, 脾, 脑

---

背景

DO1 (cysteine dioxygenase, type I) is a 200 amino acid protein that belongs to the cysteine dioxygenase family and is involved in organosulfur biosynthesis. Existing as a monomer and expressed at high levels in liver and placenta and at lower levels in brain, pancreas and heart, CDO1 functions as a dioxygenase that uses iron and zinc as cofactors to catalyze the conversion of L-cysteine and oxygen to 3-sulfinoalanine.

---

图像

WB检测Cysteine Dioxygenase CDO1蛋白(货号 GB114677). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 1500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.

---

储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

---

注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.