产品信息

蛋白质全称	丝氨酸/苏氨酸蛋白激酶Sgk3
别名	Cytokine-independent survival kinase, SGK2, Serum/glucocorticoid-regulated kinase 3, Sgkl, Serum/glucocorticoid-regulated kinase-like, Serine/threonine protein kinase Sgk3, Cisk
Uniprot ID	Q9ERE3
免疫原	小鼠SGK3重组蛋白
抗体亚型	IgG
预测分子量./观测分子量.	57 kDa / 57 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	人, 小鼠, 大鼠	1: 500-1: 1000	胰腺, 肝

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背景

Serine/threonine-protein kinase Sgk3 (SGK3), belongs to the Ser/Thr protein kinase family of proteins. The serum- and glucocorticoid-regulated kinase proteins are closely related to the Akt protein family. SGK1, a homolog of SGK3, activates ion channels, in particular potassium (K+) channels. SGK2 and SGK3 have been found to also be involved in this activation process, making all three of these proteins important regulators for cell proliferation, epithelial transport and neuromuscular excitability.

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图像

WB检测SGK3蛋白(货号 GB11983). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.