产品信息

蛋白质全称	Flap 核酸内切酶1
别名	FEN-1, Flap structure-specific endonuclease 1, Fen1, Fen-1, DNase IV, flap structure endonuclease 1, hFEN 1, Maturation factor 1, MF1, RAD2
Uniprot ID	P39749
免疫原	小鼠FEN1重组蛋白
抗体亚型	IgG
预测分子量./观测分子量.	42 kDa / 45 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	人, 小鼠	1: 500-1: 1000	肺, 睾丸

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背景

FEN1(Flap endonuclease-1) is the prototypical member of the 5’-nuclease superfamily, whose activities span a range of cellular pathways involved in DNA replication and genome maintenance . FEN1 is a structure-selective metallonuclease essential for Okazaki fragment maturation through efficient removal of 5’ flaps resulting from strand displacement during lagging-strand synthesis. FEN1 is overexpressed in multiple cancer types, and has been suggested both as a biomarker relating to prognosis and disease progression and as a potential therapeutic target.

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图像

WB检测FEN1蛋白(货号 GB111455). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.