产品信息

蛋白质全称	DNA损伤诱导转录因子3
别名	C/EBP homologous protein, C/EBP homologous protein 10, CEBPZ, CHOP 10, CHOP, GADD153, CHOP10, DDIT 3, DDIT3, Chop10, Gadd153, CHOP-10, DNA damage-inducible transcript 3, DNA damage inducible transcript 3
Uniprot ID	P35638
免疫原	KLH偶联的人CHOP合成肽
抗体亚型	IgG
预测分子量./观测分子量.	19 kDa / 27-30 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	小鼠, 大鼠	1: 500-1: 1000	衣霉素处理C2C12, 衣霉素处理C6

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背景

This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis.

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图像

WB检测DDIT3/CHOP蛋白(货号 GB115762). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 1000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.