Product Information Description AKT rabbit polyclonal Protein full name v-akt murine thymoma viral oncogene homolog 1 Synonyms AKT1, AKT, CWS6, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA,AKT serine/threonine kinase 1 Immunogen Recombinant protein corresponding to Human AKT Isotype IgG Purity Affinity purification Predicted MW. 56 kDa Observed MW. 60 kDa Uniprot ID P31749, P31750, P47196 Applications applications species dilution positive tissue WB Human, Mouse, Rat 1: 1000-1: 2000 Jurkat, MCF7, HT29, HCT116, SW480, lung Background RAC-alpha serine/threonine-protein kinase is an enzyme that in humans is encoded by the AKT1 gene. This enzyme belongs to the AKT subfamily of serine/threonine kinases. It is commonly referred to as PKB, or by both names as "Akt/PKB". The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Images Western blot analysis of AKT (GB13427) at dilution of 1: 1000 Lane 1: Jurkat cell lysate Lane 2: MCF7 cell lysate Lane 3: HT29 cell lysate Lane 4: HCT116 cell lysate Lane 5: SW480 cell lysate Lane 6: Mouse lung tissue lysate Storage Storage Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. Storage Buffer PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. NOTE: 1.This product is intended for research only. 2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （ G2025 ）.