Anti-Phospho-Smad2 (S425) Rabbit pAb
- 50 μL
Product Information
Description | Phospho-Smad2 (S425) rabbit polyclonal |
Protein full name | Mothers against decapentaplegic homolog 2 |
Synonyms | SMAD2, JV18, JV18-1, MADH2, MADR2, hMAD-2, hSMAD family member 2, p-smad2 |
Immunogen | KLH conjugated Synthetic phosphopeptide corresponding to Human smad2 around the phosphorylation site of Ser425 |
Isotype | IgG |
Purity | Affinity purification |
Predicted MW. | 52 kDa |
Observed MW. | 58 kDa |
Uniprot ID |
Applications
Applications | Species | Dilution | Positive Tissue |
WB | Mouse, Rat | 1: 500-1: 1000 | heart, muscle, liver |
Background
This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin.
Images
 |
Western blot analysis of Smad2 (phospho S425) (YB13207-2) at dilution of 1: 1000. |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （Y2036）.
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