Gene Name Synonym

PDCD1, CD279, PD-1, PD1, SLEB2, hPD-1, hPD-l, hSLE1, programmed cell death 1,PD-1,CD279,PD1,SLEB2,hPD-1,hPD-l,hSLE1,PD-1/CD279

Species

Human

Accession

Q15116

Source

HEK293 cells

SPECIFICATIONS

Amino acid sequence

Pro21-Val170 is expressed with a 10×His tag at the N terminus

Molecular Weight

16.8 kDa

Purity

>95.0% as determined by SDS-PAGE

Endotoxin

<5 EU mg^-1

Formulation

Lyophilized from sterile PBS, pH 7.4.Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization.

Reconstitution

Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation.

STORAGE

Stability and Storage

12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

BACKGROUND

Programmed cell death 1(PD-1), also known as PDCD1, is a type I transmembrane glycoprotein, and is an immunoreceptor belonging to the CD28/CTLA-4 family negatively regulates antigen receptor. PD-1 plays a vital role in inhibiting immune responses and promoting self-tolerance through modulating the activity of T-cells, activating apoptosis of antigen-specific T cells and inhibiting apoptosis of regulatory T cells. PD-1 is expressed in peripheral blood and also abundantly expressed in follicular lymphoma (FL). PD-1 and PD-L1 (also known as CD274 or B7-homolog 1 [B7-H1]) inhibitory signaling is an essential mechanism behind immune regulation of disease states, such as autoimmunity, cancer and neurodegenerative diseases.

Data

SDS-PAGE

Bioactivity

Active Recombinant Human PD1 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 30-40 kDa.

Measured by its binding ability in a functional ELISA. Immobilized human PDL1 at 3 μg/ml (100 μl /well) can bind biotinylated human PD1 with a linear range of 0.039-0.652 μg/mL.