Gene Name Synonym

CD86, B7-2, B70, CD28LG2, LAB72, MGC34413

Species

Human

Accession

P42081

Source

HEK293 cells

SPECIFICATIONS

Amino acid sequence

Ala24-Pro247 is expressed with a 10×His tag at the N terminus

Molecular Weight

25.6 kDa（The protein migrates as 40~60 kDa under reducing condition (SDS-PAGE) due to glycosylation）

Purity

>95.0% as determined by SDS-PAGE

Endotoxin

<5 EU mg^-1

Formulation

Lyophilized from sterile PBS, pH 7.4.Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization.

Reconstitution

Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation.

STORAGE

Stability and Storage

12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

BACKGROUND

CD86, also known as B-lymphocyte activation antigen B7-2，is a member of the cell surface immunoglobulin superfamily. CD86 provides a stimulatory signal to B cell function. CD86 has also been shown to be important in initiation of IL-4 responses. The B7 (CD80, CD86) molecules on APC and their ligands on T cells (CD28, CTLA4 [CD152]) provide crucial costimulation signals for the regulation of effective immune responses. CD86 has an important role in chronic hemodialysis, allergic pulmonary inflammation, arthritis, and antiviral responses.

Data

SDS-PAGE

Bioactivity

Testing in progress

Recombinant Human CD86 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 42-65 kDa.