Gene Name Synonym

CD45, PTPRC, L-CA, T200

Species

Human

Accession

P08575

Source

HEK293 cells

SPECIFICATIONS

Amino acid sequence

Gln26-Asn575 is expressed with a 10×His tag at the C terminus

Molecular Weight

60.6 kDa

Purity

>95.0% as determined by SDS-PAGE

Endotoxin

<5 EU mg^-1

Formulation

Lyophilized from sterile PBS, pH 7.4.Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization.

Reconstitution

Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation.

STORAGE

Stability and Storage

12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

BACKGROUND

Protein tyrosine phosphatase, receptor type C (CD45), also known as PTPRC is a member of the protein tyrosine phosphatase (PTP) family which is known for its function to serve as signaling molecules and to regulate a variety of cellular processes. CD45, the first and prototypic receptor-like protein tyrosine phosphatase, is expressed on all nucleated hematopoietic cells. CD45, is one of the key players in the initiation of T cell receptor signaling by controlling the activation of the Src family protein-tyrosine kinases Lck and Fyn. CD45 deficiency results in T-and B-lymphocyte dysfunction in the form of severe combined immune deficiency. Expression of CD45 is essential for the activation of T cells via the TCR, and that different CD45 isoforms display a different ability to support T cell activation.

Data

SDS-PAGE

Bioactivity

Testing in progress.

Recombinant Human CD45 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 120-140 kDa.