产品信息

蛋白质全称	cAMP/cGMP 3',5'-环磷酸二酯酶10A
别名	Pde10a, 5''-cyclic phosphodiesterase 10A, cAMP and cAMP-inhibited cGMP 3'', FLJ11894, Phosphodiesterase 10A, Phosphodiesterase 10A1
Uniprot ID	Q8CA95
免疫原	小鼠PDE10A重组蛋白
抗体亚型	IgG
预测分子量./观测分子量.	89 kDa / 75 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	小鼠, 大鼠	1: 500-1: 1000	脑, 睾丸

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背景

The PDE10A plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides，eliminate cAMP- and cGMP-mediated intracellular signaling by hydrolyzing the cyclic nucleotide to the corresponding nucleoside 5-prime monophosphate. Can hydrolyze both cAMP and cGMP, but has higher affinity for cAMP and is more efficient with cAMP as substrate.

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图像

WB检测PDE10A蛋白(货号 YB111431). 样品: 经RIPA裂解液(货号Y2013)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号YC310012)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号YB23314), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(Y2036)稀释本产品.