产品信息

蛋白质全称	丝氨酸消旋酶
别名	D-serine ammonia-lyase, L-serine ammonia-lyase, D-serine dehydratase, L-serine dehydratase, Srr, ISO1, ILV1
Uniprot ID	Q9QZX7
免疫原	小鼠Serine racemase重组蛋白
抗体亚型	IgG
预测分子量./观测分子量.	36 kDa / 36 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	小鼠, 大鼠	1: 500-1: 1000	脑, 肝

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背景

Serine racemase is a member of the family of pyridoxal-5’ phosphate-dependent enzymes and is localized to glial cells in rat brain. Known to be prominent in bacteria, D amino acids were generally thought to be absent in mammals. D-serine has since been found in high levels in the mammalian brain and in various mammalian fluids. D-serine activates N-methyl-D-aspartate (NMDA) receptors--molecules with important roles in learning, brain growth and brain cell death. Serine racemase is the enzyme catalyzing the formation of D-serine from L-serine.

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图像

WB检测Serine racemase蛋白(货号 YB112077). 样品: 经RIPA裂解液(货号Y2013)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号YC310012)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号YB23314), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(Y2036)稀释本产品.