产品信息

蛋白质全称	Rho 鸟嘌呤核苷酸交换因子 1
别名	Lbc's second cousin, Lymphoid blast crisis-like 2, Arhgef1, Lbcl2, Lsc, 115 kDa guanine nucleotide exchange factor, GEF1, Lsc homolog, p115RhoGEF, LBCL2LSC
Uniprot ID	Q92888
免疫原	KLH偶联的人p115-RhoGEF合成肽
抗体亚型	IgG
预测分子量./观测分子量.	103 kDa / 80 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	人	1: 1000-1: 2000	HeLa细胞, 293T细胞, A549细胞

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背景

This protein seems to play a role in the regulation of RhoA GTPase by guanine nucleotide binding alpha-12 (GNA12) and alpha-13 (GNA13) subunits. Acts as GTPase-activating protein (GAP) for GNA12 and GNA13, and as guanine nucleotide exchange factor (GEF) for RhoA GTPase. Activated G alpha 13/GNA13 stimulates the RhoGEF activity through interaction with the RGS-like domain. This GEF activity is inhibited by binding to activated GNA12. Mediates angiotensin-2-induced RhoA activation.

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图像

WB检测p115-RhoGEF蛋白(货号 YB112391). 样品: 经RIPA裂解液(货号Y2013)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号YC310012)溶于TBST溶液, 室温孵育1小时. —抗: 1: 1000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号YB23314), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(Y2036)稀释本产品.