产品信息

蛋白质全称	周期素依赖性样激酶5
别名	CDK5, Cell division protein kinase 5, cyclin dependent kinase 5, PSSALRE, TPKII catalytic subunit
Uniprot ID	P49615
免疫原	KLH偶联的小鼠CDK5合成磷酸肽
抗体亚型	IgG
预测分子量./观测分子量.	33 kDa / 33,30 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	人	1: 300-1: 400	SHSY-5Y细胞

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背景

Proline-directed serine/threonine-protein kinase essential for neuronal cell cycle arrest and differentiation and may be involved in apoptotic cell death in neuronal diseases by triggering abortive cell cycle re-entry. Interacts with D1 and D3-type G1 cyclins.Phosphorylates SRC, NOS3, VIM/vimentin, p35/CDK5R1, MEF2A, SIPA1L1, SH3GLB1, PXN, PAK1, MCAM/MUC18,SEPT5,SYN1,DNM1,AMPH,SYNJ1,CDK16, RAC1,RHOA,CDC42,TONEBP/NFAT5,MAPT/TAU,MAP1B, histone H1,p53/TP53,HDAC1,APEX1, PTK2/FAK1, huntingtin/HTT, ATM, MAP2, NEFH and NEFM. Regulates several neuronal development and physiological processes including neuronal survival,migration and differentiation, axonal and neurite growth,synaptogenesis,oligodendrocyte differentiation, synaptic plasticity and neurotransmission, by phosphorylating key proteins.

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图像

WB检测CDK5蛋白(货号 YB115474). 样品: 经RIPA裂解液(货号Y2013)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号YC310012)溶于TBST溶液, 室温孵育1小时. —抗: 1: 300稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号YB23314), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(Y2036)稀释本产品.