Gene Name Synonym

IGF-I, IGF-IA, Mechano growth factor, MGF, Somatomedin C

Species

Human

Accession

P05019

Source

E.coli

SPECIFICATIONS

Amino acid sequence

E.coli-derived Human IGF-1 protein, Gly49-Ala118.

Molecular Weight

Approximately 8.0 kDa.

Purity

>97.0% as determined by SDS-PAGE.

Endotoxin

<1.0 EU/μg of the protein by the LAL method.

Formulation

Lyophilized from sterile Tris-Hcl, pH 8.0. Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization.

Reconstitution

Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation.

STORAGE

Stability and Storage

12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

BACKGROUND

Insulin-like Growth Factor I (IGF-I), also known as Mechano Growth Factor, somatomedin-C, IGF-I, and IGF1, is a secreted protein that belongs to the insulin family which stimulates the proliferation and survival of various cell types including muscle, bone, and cartilage tissue in vitro. IGF1 is the dominant effector of Growth Hormone (GH) and is structurally homologous to Proinsulin. The production of IGF-1 is stimulated by growth hormone (GH) and can be retarded by undernutrition, growth hormone insensitivity, lack of growth hormone receptors, or failures of the downstream signaling pathway post GH receptor including SHP2 and STAT5B. IGF-I is a mitogenic cytokine, binds to IGF type 1 receptor, and modulates growth in many tissues, such as nervous tissue, lymphoid tissue, reproductive tissue, smooth muscle, endothelium, and bone. IGF1 also mediates neuroprotective mechanism and has been shown to enhance neuronal survival and inhibit apoptosis. The body growth of animals is regulated by growth hormone and IGF-I. The classical theory of this regulation is that most IGF-I in the blood originates in the liver and that body growth is controlled by the concentration of IGF-I in the blood. Meanwhile, IGF-1 promote the proliferation of multiple myeloma (MM) cells and protect them against dexamethasone (Dex)-induced apoptosis. Mature human IGF-I shares 94% and 96% amino acid (aa) sequence identity with the mouse and rat orthologs, respectively.

Data

SDS-PAGE

Bioactivity

Recombinant Human IGF-1 Protein was determined by SDS-PAGE with Coomassie Blue.

Measured in a cell proliferation assay using PDC-P1 cells. The ED50 for this effect is 0.01-0.1 μg/mL.