Gene Name Synonym

CSF-1, MGI-IM, MCSF, M-CSF

Species

Human

Accession

P09603-3

Source

E.coli

SPECIFICATIONS

Amino acid sequence

E.coli-derived Human M-CSF protein, Glu33-Ser190.

Molecular Weight

Approximately 18.5 kDa.

Purity

>95.0% as determined by SDS-PAGE.

Endotoxin

<1.0 EU/μg of the protein by the LAL method.

Formulation

Lyophilized from sterile Tris-Hcl, pH 8.0. Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization.

Reconstitution

Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation.

STORAGE

Stability and Storage

12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

BACKGROUND

Macrophage colony-stimulating factor 1, also known as CSF-1, M-CSF, Lanimostim and CSF1, is a single-pass membrane protein which is disulfide-linked as a homodimer or heterodimer.  M-CSF is a proinflammatory cytokine, constitutively produced by several cell types, such as fibroblasts, endothelial cells, stromal cells, macrophages, smooth muscle cells and osteoblasts, binds to its receptor CSF1R, and exists in several isoforms as a secreted glycoprotein, a cell-surface protein and a proteoglycan. M-CSF/CSF-1 is a secreted cytokine which influences hemopoietic stem cells to differentiate into macrophages or other related cell types. M-CSF is involved in the development and proliferation of cells of the monocyte/macrophage lineage and participates in the induction of osteoclasts, which are important in the destruction of bone and cartilage and in the periarticular osteoporotic changes seen in patients with rheumatoid arthritis. M-CSF Induces vascular endothelial growth factor production and angiogenic activityfrom human monocytes. Whatmore, M-CSF and GM-CSF are mediators involvedin regulating the numbersand function of macrophagelineage populations and have been shown to contribute to macrophage heterogeneity.

Data

SDS-PAGE

Bioactivity

Recombinant Human M-CSF Protein was determined by SDS-PAGE with Coomassie Blue.

Measured in a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is 5-10 ng/mL.