Gene Name Synonym

CSF2, GM-CSF, MGC131935

Species

Human

Accession

P04141

Source

CHO cells

SPECIFICATIONS

Amino acid sequence

Ala18-Glu144 is expressed with a 10×His tag at the N terminus

Molecular Weight

15.9 kDa

Purity

>95.0% as determined by SDS-PAGE

Endotoxin

<5 EU·mg^-1

Formulation

Lyophilized from sterile PBS, pH 7.4.Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization.

Reconstitution

Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation.

STORAGE

Stability and Storage

12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

BACKGROUND

Colony-stimulating factor 2 (CSF-2) is a hematopoietic growth factor involved in the differentiation of various myeloid cells from hematopoietic progenitor cells. GM-CSF has been shown to play a crucial role in the pathogenesis of the experimental autoimmune encephalomyelitis (EAE), an animal model for multiple sclerosis. GM-CSF is a major cytokine produced by the encephalitogenic IL-17-producing T helper cells (Th17 cells) and required for Th17 cell-mediated EAE pathogenesis. GM-CSF stimulates the differentiation of hematopoietic progenitors to monocytes and neutrophils, and reduces the risk for febrile neutropenia in cancer patients. GM-CSF has been utilized in the clinical management of multiple disease processes. Most recently, GM-CSF has been incorporated into the treatment of malignancies as a sole therapy, as well as a vaccine adjuvant.

Data

SDS-PAGE

Bioactivity

Active Recombinant Human CSF2 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 25-30 kDa.

CSF-2 stimulates the proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.1-0.5 ng/ml.