Synonym

IL-23 alpha & IL-12 beta,CLMF,CLMF2,NKSF,NKSF2

Species

Human

Accession

Q9NPF7&P29460

Source

HEK293 cells

SPECIFICATIONS

Amino acid sequence

Arg20-Pro189&Ile23-Ser328 is expressed with a 10×His tag at the C terminus

Molecular Weight

20.1 kDa+36.1kDa

Purity

>95.0% as determined by SDS-PAGE

Endotoxin

<5 EU·mg^-1

Formulation

Lyophilized from sterile PBS, pH 7.4.Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization.

Reconstitution

Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation.

STORAGE

Stability and Storage

12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

BACKGROUND

Interleukin 23 (IL-23) is a member of the IL-12 cytokine family and composed of two subunits, IL-12p40 and IL-23p19. heterodimeric cytokine which functions in innate and adaptive immunity.  IL-23 binds to an heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. The main biological effects of IL-23 identified initially consist of stimulation of antigen presentation by DCs, T cell differentiation to Th17 cells, and production of interferon-γ (IFN-γ). IL-23 acts also as an end-stage effector cytokine through direct action on macrophages.

Data

SDS-PAGE

Bioactivity

Active Recombinant Human IL-23 Protein was determined by SDS-PAGE with Coomassie Blue.