Gene Name Synonym

Tnni3; Troponin I; cardiac muscle; Cardiac troponin I

Species

Rat

Accession

P23693

Source

HEK293 cells

SPECIFICATIONS

Amino acid sequence

Ala2-Gly211 is expressed with a 10×His tag at the N terminus

Molecular Weight

15.2 kDa

Purity

>95.0% as determined by SDS-PAGE

Endotoxin

<5 EU mg^-1

Formulation

Lyophilized from sterile PBS, pH 7.4. Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization.

Reconstitution

Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation.

STORAGE

Stability and Storage

12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

BACKGROUND

Tnni3 is the inhibitory subunit of the cardiac troponin (cTn) complex that is a sensor of the intracellular Ca2+ levels and modulates the interaction between the thick and thin filaments during muscle contraction. It mainly blocks the interaction between actin and myosin in the absence of Ca2+ and tightly regulated by phosphorylation.

Data

SDS-PAGE

Bioactivity

Recombinant Rat TNNI3 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 11-15 kDa.

Immobilized Rat TNNI3, His Tag at 3 μg/mL (100 μL/well) can bind Anti-Cardiac Troponin I with a linear range of 0.009-0.078 μg/mL.