产品信息

蛋白质全称	活化T细胞核因子胞质3
别名	NF-ATc3, NFATc3, NFATx, NFATC3, NFAT4, T-cell transcription factor NFAT4, NF-AT4, NF-AT4c
Uniprot ID	Q12968
免疫原	小鼠NFAT4/NF-ATc3重组蛋白
抗体亚型	IgG
纯化方式	亲和纯化
亚细胞定位	细胞质, 细胞核

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应用

应用	物种	稀释	阳性样品
IHC/IF 免疫组织化学/免疫荧光	人	1: 250-1: 2000	结肠癌, 结肠

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背景

NFATC3 (nuclear factors of activated T cells) proteins are a family of transcription factors originally identified as mediators of activation of cytokine genes in response to antigenic stimulation of T cells.NFATC3 is widely expressed being found in DP thymocytes, CD4+ T cells, smooth muscle cells, Schwann cells and mast cells. NFATC3 regulates gene transcription as part of a NFATC transcription complex. It is normally cytoplasmic and phosphorylated. Upon a rise in intracellular Ca+2, dephosphorylation occurs via calcineurin, and NFATC3 enters the nucleus. Human NFATC3 is 1075 amino acids in length. It contains a calcineurin binding site (aa 109‑114), two nuclear localization sites (aa 273‑275 and 686‑688), one nuclear export site (a 1032‑1041) and an RH domain that binds DNA (aa 433‑592).

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图像

	IHC检测NFAT4/NF-ATc3蛋白(货号 YB111039). 样品: 人结肠癌, 4%多聚甲醛 (货号Y1112) 固定12-24小时. 抗原修复: 柠檬酸抗原修复液(干粉, pH 6.0) (Y1212), 98℃, 20分钟. —抗: 1: 1100稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号YB23314), 1: 200稀释, 室温孵育1小时.
	IHC检测NFAT4/NF-ATc3蛋白(货号 YB111039). 样品: 人结肠, 4%多聚甲醛 (货号Y1112) 固定12-24小时. 抗原修复: 柠檬酸抗原修复液(干粉, pH 6.0) (Y1212), 98℃, 20分钟. —抗: 1: 1100稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号YB23314), 1: 200稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(Y2036)稀释本产品.