产品信息

蛋白质全称	SWI/SNF 相关、基质相关、肌动蛋白依赖性调节因子亚家族 E 成员 1 相关
别名	SMARCE1-related protein, BRCA2-associated factor 35, HMG box-containing protein 20B, Structural DNA-binding protein BRAF35, Hmg20b, Braf35, Hmgx2, Smarce1r
Uniprot ID	Q9Z104
免疫原	小鼠BRAF35重组蛋白
抗体亚型	IgG
预测分子量./观测分子量.	36 kDa / 36 kDa
纯化方式	亲和纯化

---

应用

应用	物种	稀释	阳性样品
WB 免疫印迹	人	1: 1000-1: 2000	293T细胞, A375细胞, Jurkat细胞

---

背景

CoREST (corepressor of repressor element-1-silencing transcription factor) is a chromatin- modifying complex that was first identified as a regulator of neuronal gene expression, having a essential role in neuronal differentiation . The CoREST complex is recruited by REST (RE1-silencing transcription factor) that recognizes the 21-nucleotide RE-1 sequence in the promoter of more than 1,700 different target genes in human and mouse . HMGB20B is one component of the CoREST complex, which mediates chromatin remodeling and repression of specific genes involved in neuronal differentiation through modulation of chromatin structure.

---

图像

WB检测BRAF35蛋白(货号 YB112991). 样品: 经RIPA裂解液(货号Y2013)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号YC310012)溶于TBST溶液, 室温孵育1小时. —抗: 1: 2000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号YB23314), 1: 5000稀释, 室温孵育1小时.

---

储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

---

注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(Y2036)稀释本产品.