产品信息

蛋白质全称	叉头框蛋白L2
别名	Pituitary forkhead factor, P-Frk, Foxl2, Pfrk, BPES1, Blepharophimosis, PINTO, POF3
Uniprot ID	O88470
免疫原	小鼠FOXL2重组蛋白
抗体亚型	IgG
纯化方式	亲和纯化
亚细胞定位	细胞核

---

应用

应用	物种	稀释	阳性样品
IHC/IF 免疫组织化学/免疫荧光	小鼠	1: 3000-1: 6000	卵巢

---

背景

Transcriptional regulator. Critical factor essential for ovary differentiation and maintenance, and repression of the genetic program for somatic testis determination. Prevents trans-differentiation of ovary to testis throught transcriptional repression of the Sertoli cell-promoting gene SOX9 (By similarity). Has apoptotic activity in ovarian cells. Suppresses ESR1-mediated transcription of PTGS2/COX2 stimulated by tamoxifen (By similarity). Is a regulator of CYP19 expression (By similarity). Participates in SMAD3-dependent transcription of FST via the intronic SMAD-binding element (By similarity). Is a transcriptional repressor of STAR.

---

图像

IHC检测FOXL2蛋白(货号 YB113538). 样品: 小鼠卵巢, 4%多聚甲醛 (货号Y1112) 固定12-24小时. 抗原修复: 柠檬酸抗原修复液(干粉, pH 6.0) (Y1212), 98℃, 20分钟. —抗: 1: 4000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号YB23314), 1: 200稀释, 室温孵育1小时.

---

储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

---

注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(Y2036)稀释本产品.