Anti-TNF-alpha antibody (HRP Conjugated)
- 50 μL
产品信息
蛋白质全称 | 肿瘤坏死因子α |
别名 | TNF,DIF,TNF-alpha,TNFA,TNFSF2,Tumour necrosis factor, Tumour necrosis factor alpha,tumor necrosis factor, TNLG1, TNF-α, TNF a, TNF alpha,NFα, TNF-α, Cachectin, TNFα |
Uniprot ID | P01375 |
免疫原 | 人TNF-alpha重组蛋白 |
预测分子量./观测分子量. | 26 kDa / 25/28 kDa |
纯化方式 | 亲和纯化 |
偶联 | 辣根过氧化物酶 |
应用
| 应用 | 物种 | 稀释 | 阳性样品 |
| WB 免疫印迹 | 小鼠 | 1: 500-1: 1000 | RAW264.7细胞 |
| 孵化条件 | 封闭后, 将膜与HRP标记的抗TNF-alpha 抗体(YB115737-HRP)在室温（20-25℃）孵育3-4小时或4℃过夜, 随后用TBST缓冲液洗涤3次, 每次5分钟. | ||
背景
TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR.
图像
| WB检测TNF-alpha蛋白(货号 YB115737-HRP). 样品: 经RIPA裂解液(货号Y2013)处理的蛋白质. 封闭: 3% BSA（货号YC305021）的PBS溶液, 室温孵育30分钟. 抗体: HRP标记的抗TNF-alpha蛋白抗体 1: 500稀释, 4℃ 孵育过夜. |
储存
| 储存条件 | 在-20℃下储存一年, 避免反复冻融. |
| 储存缓冲液 | 含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.产品颜色为浅黄色. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （Y2036）.
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