产品信息

蛋白质全称	5'-AMP活化蛋白激酶β1亚基
别名	AMPK subunit beta-1, AMPKb, Prkab1, AMPK, AMPK-BETA-1, HAMPKb, AMP-activated, AMPK, Prkab1, PRKAB1, protein kinase AMP-activated non-catalytic subunit beta 1, AMPKβ1
Uniprot ID	Q9Y478
免疫原	人AMPK beta 1重组蛋白
抗体亚型	IgG2b, κ
克隆号	7E4H5
预测分子量./观测分子量.	30 kDa / 38 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	人, 小鼠, 大鼠	1: 500-1: 1000	结肠, 肺, 脑

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背景

AMPK beta 1 is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol.

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图像

WB检测AMPK beta 1蛋白(货号 YB12638). 样品: 经RIPA裂解液(货号Y2013)处理的蛋白质. 封闭: 3%脱脂牛奶(货号YC310012)溶于TBST溶液, 室温孵育1小时. —抗: 1: 1000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗小鼠IgG (H+L) (货号YB23312), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(Y2036)稀释本产品.